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rat monoclonal anti zo 1  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank rat monoclonal anti zo 1
    Rat Monoclonal Anti Zo 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+monoclonal+anti+zo+1/anti-ZO-1/bio_rxiv__64898__2026__04__13__717734-161-60-66
    Average 95 stars, based on 43 article reviews
    rat monoclonal anti zo 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Microtubule binding protein Togaram1 is required for proper development of mammalian forebrain and neural primary cilia
    Article Snippet: Slabs were then washed twice with PBS and coverslipped with VectaShield fluorescent mounting medium (Vector Laboratories Inc., H-1000).

    Bioprocessing:

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions.
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Hemagglutination Assay:

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Generated:

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions.
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Cell Biology:

    Article Title: Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions
    Article Snippet: .. For the blots, we employed mouse monoclonal antibodies against HA (Cat. H3663, Sigma Aldrich, St. Louis, MO, USA), FLAG (Cat. F1804; dilution 1:5000, Sigma-Aldrich, St Louis, MO, USA), occludin (Cat. 33-1500; dilution 1:1000, Invitrogen, Camarillo, CA, USA) and actin (generated and generously provided by Dr. Manuel Hernández, Cinvestav, Department of Cell Biology), and a rat monoclonal anti-ZO-1 (Cat. R26.4C, dilution 1:500; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA). .. As secondary antibodies, we employed peroxidase-conjugated goat anti-mouse IgG (Cat. 626520, dilution 1:10,000 Invitrogen, Camarillo, CA, USA) and anti-rat IgG (Cat. 81-9520, dilution 1:10,000, Invitrogen, Camarillo, CA, USA), followed by Immobilon chemiluminescence detection (Cat. WBKLS 0500, Darmstadt, Germany).

    Labeling:

    Article Title: Loss of gap junction plaques and inhibition of intercellular communication in ilimaquinone-treated BICR-M1Rk and NRK cells.
    Article Snippet: To examine the mechanism(s) and pathways of gap junction formation and removal a novel and reversible inhibitor of protein secretion, ilimaquinone (IQ), was employed.. IQ has been reported to cause the vesiculation of Golgi membranes, block protein transport at the cis-Golgi and depolymerize cytoplasmic microtubules.. Connexin43 (Cx43) immunolabeling and dye microinjection experiments revealed that gap junction plaques were lost and intercellular communication was inhibited following IQ treatment for 1 hr in BICR-M1Rk rat mammary tumor cells and for 2 hr in normal rat kidney (NRK) cells.



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